Name: Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein, Ultra Low Endotoxin (MHC-HM405T-UL)
Brand: KactusBio
SKU: MHC-HM405T-UL-100UG
Price: 908.0 USD
Availability: InStock

Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein, Ultra Low Endotoxin (MHC-HM405T-UL)

Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag with an affinity constant of 0.09 nM as determined in SPR assay (Biacore T200).

Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA (QC Test).

Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer was greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide

Catalog #MHC-HM405T-UL

Size

100UG

500UG

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Product Description	Recombinant Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and SLLMWITQC peptide.
Product Background	NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein	HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC)
Synonyms	MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1; CTAG1A
MHC Form	Tetramer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM PB, 500mM NaCl (pH 7.4). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag with an affinity constant of 0.09 nM as determined in SPR assay (Biacore T200).
	Immobilized Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA (QC Test).
	Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
	The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer was greater than 95% as determined by SEC-HPLC.