Product Description |
Recombinant Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and HMTEVVRHC peptide. |
Product Background |
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide |
Molecular Weight |
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
Activity |
The affinity constant of 2.35 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag with an affinity constant of 2.35 nM as determined in SPR assay (Biacore T200). |
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Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer is greater than 95% as determined by SEC-HPLC. |
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