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Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein (MHC-HE027) Summary
Product Description |
Recombinant Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAA peptide. |
Product Background |
A key regulator of cell division is polo-like kinase 1 (PLK1), a member of the serine/threonine kinase family that plays an important role in regulating the mitotic and meiotic cell cycle. The phosphorylation of specific substrates mediated by PLK1 controls nuclear envelope breakdown (NEBD), centrosome maturation, proper spindle assembly, chromosome segregation, and cytokinesis. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Specifications
Protein |
HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) |
Synonyms |
PLK-1; STPK13; PLK |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&AAPGAPAAA |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAA peptide |
Molecular Weight |
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.7ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.7ng/ml determined by ELISA. |
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Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer is greater than 95% as determined by SEC-HPLC. |
Additional Information
Other Available Proteins:
Finding available proteins...
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