Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein (MHC-HE045)
  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide
Catalog #MHC-HE045
Regular price $341.00
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Product Description

Recombinant Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide.

Product Background

PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein

HLA-A*02:01&B2M&PRAME (SLLQHLIGL)

Synonyms

PRAME; OIP-4; OIP4; MAPE;

MHC Form

Monomer

Allele

HLA-A*02:01

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide

Molecular Weight

The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.

Activity

Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

E.coli

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC.

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