- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human HLA-A*02:01&B2M&TdT (ALYDKTKRI)-Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.3ng/ml determined by ELISA.
- Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYDKTKRI peptide
Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein (MHC-HM486) Summary
Product Description |
Recombinant Human HLA-A*02:01&B2M&TdT (ALYDKTKRI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYDKTKRI peptide. |
Product Background |
The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3'-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&TdT (ALYDKTKRI) Specifications
Protein |
HLA-A*02:01&B2M&TdT (ALYDKTKRI) |
Synonyms |
DNA nucleotidylexotransferase; terminal deoxynucleotidyl transferase |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&ALYDKTKRI |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYDKTKRI peptide |
Molecular Weight |
The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-A*02:01&B2M&TdT (ALYDKTKRI)-Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.3ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
Additional Information
Other Available Proteins:
Finding available proteins...
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