• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and TLIRWAILEA peptide
Catalog #MHC-HE041
Price
Regular price $34100
Regular price Sale price $34100
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Size
Avantor VWR Fisher Scientific

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Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein (MHC-HE041) Summary

Product Description

 Recombinant Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and TLIRWAILEA peptide.

Product Background

 TLIRWAILEA is position 344-353 of Foxp3 which is a member of a large forkhead family of related proteins. The peptide has minimal homology with other Foxp family members, such as Foxp1, 2 and 4, and it has also been shown to induce strong peptide-specific CD8+ T cell responses, which recognize Foxp3+/HLA-A*02:01+ cutaneous T lymphoma cells.

HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Specifications

Protein
 HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA)
Synonyms
 TLIRWAILEA; Foxp3
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and TLIRWAILEA peptide
Molecular Weight
 The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity
 Immobilized Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.2ng/ml determined by ELISA. Immobilized Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer is greater than 95% as determined by SEC-HPLC.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
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