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Human HLA-A*02:01&B2M&Intersectin-2 (YQNKLIYLV) Monomer Protein (MHC-HE018) Summary
Product Description |
Recombinant Human HLA-A*02:01&B2M&Intersectin-2 (YQNKLIYLV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YQNKLIYLV peptide. |
Product Background |
Intersectin-2 (ITSN2) is an evolutionarily conserved scaffold protein that plays a role in endocytic internalization, regulation of the actin cytoskeleton, and epithelial morphogenesis. It is involved in the initiation stage of clathrin-mediated endocytosis and has been implicated in distinct pathologies. Alternative splicing generates several isoforms of ITSN2. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&Intersectin-2 (YQNKLIYLV) Specifications
Protein |
HLA-A*02:01&B2M&Intersectin-2 (YQNKLIYLV) |
Synonyms |
YQNKLIYLV; Intersectin-2; SH3P18; ITSN2; KIAA1256; SH3D1B; SWAP |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&YQNKLIYLV |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YQNKLIYLV peptide |
Molecular Weight |
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-A*02:01&B2M&Intersectin-2 (YQNKLIYLV) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.5ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human HLA-A*02:01&B2M&Intersectin-2 (YQNKLIYLV) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.5ng/ml determined by ELISA (QC Test). |
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Human HLA-A*02:01&B2M&Intersectin-2 (YQNKLIYLV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human HLA-A*02:01&B2M&Intersectin-2 (YQNKLIYLV) Monomer is greater than 95% as determined by SEC-HPLC. |
Additional Information
Other Available Proteins:
Finding available proteins...
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