Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer Protein, Ultra Low Endotoxin (MHC-HM432T-UL)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide
Catalog #MHC-HM432T-UL
Regular price $908.00
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Product Description

Recombinant Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide.

Product Background

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein

HLA-A*02:03&B2M&AFP (FMNKFIYEI)

Synonyms

Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC

MHC Form

Tetramer

Allele

HLA-A*02:03

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide

Molecular Weight

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%. Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer is greater than 95% as determined by SEC-HPLC.

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