Regular price
$341.00
Order by 4pm ET for delivery on:
Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein (MHC-HM4A2) Summary
Product Description |
Recombinant Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide. |
Product Background |
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs | ADC targets |
HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Specifications
Protein |
HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) |
Synonyms |
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
MHC Form |
Monomer |
Allele |
HLA-A*11:01 |
Accession |
AAV53343.1(HLA-A*11:01)&P61769(B2M)&KITDFGRAK |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide |
Molecular Weight |
The protein has a predicted MW of 50.44 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*11:01&B2M&EGFR TCR with the EC50 of 0.49ug/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*11:01&B2M&EGFR TCR with the EC50 of 0.49ug/ml determined by ELISA (QC Test). |
|
|
Immobilized Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 16.4ng/ml determined by ELISA. |
|
|
Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer is greater than 95% as determined by SEC-HPLC. |
Additional Information
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
