Product Description |
Recombinant Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide. |
Product Background |
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. |
Product Category |
Recombinant Protein |
Target |
HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Tetramer |
Synonyms |
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
MHC Form |
Tetramer |
Allele |
HLA-A*11:01 |
Accession |
AAV53343.1(HLA-A*11:01)&P61769(B2M)&KITDFGRAK |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide |
Molecular Weight |
The protein has a predicted MW of 201.60 kDa. Due to glycosylation, the protein migrates to 205-220 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Tetramer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 50.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Tetramer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 50.3ng/ml determined by ELISA. |
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Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Tetramer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Tetramer is greater than 95% as determined by SEC-HPLC. |
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