Name: Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein (MHC-HM466)
Brand: KactusBio
SKU: MHC-HM466-100UG
Price: 310.0 USD
Availability: InStock

Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein (MHC-HM466)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and GGDPHLPTL peptide

Catalog #MHC-HM466

Size

100UG

500UG

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Product Description	Recombinant Human HLA-E01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-terminus.It contains Gly22-Thr302(HLA-E01:03), Ile21-Met119 (B2M) and GGDPHLPTL peptide.
Product Background	Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is expressed in germinal-center-derived, mononuclear Hodgkin (H) and multinuclear, diagnostic Reed-Sternberg (RS) cells in classical EBV-positive Hodgkin's lymphoma (cHL). LMP1 expression in EBV-negative H-cell lines results in a significantly increased number of RS cells.
Product Category	Recombinant Protein

Target	HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer
MHC Form	Monomer
Allele	HLA-E*01:03
Accession	P13747(HLA-E*01:03)&P61769(B2M)&GGDPHLPTL
Species	Human
Biotinylated	no
Amino Acid Range	Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and GGDPHLPTL peptide
Molecular Weight	The protein has a predicted MW of 50.15 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer is greater than 95% as determined by SEC-HPLC.

Product Resources

Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents

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