Catalog #MHC-HM466
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Regular price $34100
Regular price Sale price $34100
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Avantor VWR Fisher Scientific

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Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein (MHC-HM466) Summary

Product Description

 Recombinant Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-terminus.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119 (B2M) and GGDPHLPTL peptide.

Product Background

 Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is expressed in germinal-center-derived, mononuclear Hodgkin (H) and multinuclear, diagnostic Reed-Sternberg (RS) cells in classical EBV-positive Hodgkin's lymphoma (cHL). LMP1 expression in EBV-negative H-cell lines results in a significantly increased number of RS cells.

HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Specifications

Protein
 HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL)
Synonyms
 EBV LMP1; GGDPHLPTL
MHC Form
 Monomer
Allele
 HLA-E*01:03
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and GGDPHLPTL peptide
Molecular Weight
 The protein has a predicted MW of 50.15 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer is greater than 95% as determined by SEC-HPLC.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
Other Available Proteins:
Finding available proteins...