Product Description |
Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-Terminus.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide. |
Product Background |
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes. |
Product Category |
Recombinant Protein |
Target |
HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer |
Synonyms |
HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1 |
MHC Form |
Monomer |
Allele |
HLA-E*01:03 |
Accession |
P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide |
Molecular Weight |
The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). |
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Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer is greater than 95% as determined by SEC-HPLC. |
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