Human HLA-E*01:03 Tetramer Protein (MHC-HM406T)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
  • Activity: The affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M)and VMAPRTLVL peptide

Catalog: MHC-HM406T

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Product Description Recombinant Human HLA-E*01:03 Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M) and VMAPRTLVL peptide.
Product Category Recombinant Protein
Target HLA-E*01:03 Tetramer
Synonyms HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
Gene/Protein ID P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL
Species Human
Exact Sequence Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M)and VMAPRTLVL peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity The affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200).
Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Tetramer, His Tag with an affinity constant of 1.65 μM as determined in SPR assay (Biacore T200).
The purity of Human HLA-E*01:03 Tetramer is greater than 95% as determined by SEC-HPLC.
Human HLA-E*01:03 Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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