Human HLA-E*01:03 Tetramer Protein (MHC-HM406T)
Catalog: MHC-HM406T
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| Product Description | Recombinant Human HLA-E*01:03 Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M) and VMAPRTLVL peptide. |
| Product Category | Recombinant Protein |
| Target | HLA-E*01:03 Tetramer |
| Synonyms | HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1 |
| Gene/Protein ID | P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL |
| Species | Human |
| Exact Sequence | Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M)and VMAPRTLVL peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). |
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Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Tetramer, His Tag with an affinity constant of 1.65 μM as determined in SPR assay (Biacore T200). |
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The purity of Human HLA-E*01:03 Tetramer is greater than 95% as determined by SEC-HPLC. |
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Human HLA-E*01:03 Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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