Human HPN/Hepsin Protein (active form) (HPN-HM101)

Immobilized Human HPN, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.

Human HPN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human HPN is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HPN, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave-tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is >20000 pmol/min/ug.
Amino Acid Range: Arg45-Leu417 (D161E, R16K)

Catalog #HPN-HM101

Price

$330⁰⁰

Size

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Human HPN/Hepsin Protein (active form) (HPN-HM101) Summary

Product Description

Recombinant Human HPN/Hepsin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg45-Leu417 (D161E, R16K).

Product Background

Home parenteral nutrition (HPN) has been proposed as the treatment of choice in patients suffering from intestinal failure (IF) and has been claimed to improve survival and quality of life either in patients with benign disorders or even in those with malignancies. HPN seems to have beneficial effect but it should be considered with caution by the physicians who should take into account the indications of each patient to receive parenteral nutrition, the underlying disease and prognosis and the access of each patient to home care services.

Human HPN/Hepsin Protein (active form) (HPN-HM101) Specifications/Details

Protein	HPN/Hepsin
Synonyms	Serine protease hepsin; TMPRSS1; Hepsin
Accession	P05981
Species	Human
Biotinylated	no
Amino Acid Range	Arg45-Leu417 (D161E, R16K)
Molecular Weight	The protein has a predicted MW of 42 kDa. Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human HPN, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave-tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is >20000 pmol/min/ug.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human HPN, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
	Human HPN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human HPN is greater than 95% as determined by SEC-HPLC.