Product Description |
Recombinant Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KLPQLCTEL peptide. |
Product Background |
Human papillomavirus (HPV) 16 infection is a necessary condition for the pathogenesis and development of cervical cancer. The E6 protein is expressed by the HPV16 E6 gene and promotes malignant phenotype transformation, which is an important mechanism for the occurrence and development of cervical cancer. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KLPQLCTEL peptide |
Molecular Weight |
The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human HLA-A*02:01&B2M&HPV 16 E6 (KLPQLCTEL) Monomer is greater than 95% as determined by SEC-HPLC. |
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