Human HPX Protein (HPX-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Thr24-His462

Catalog: HPX-HM101

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Product Description Recombinant Human HPX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr24-His462.

Product Background

Hemopexin (HPX) serves as scavenger and transporter of toxic plasma heme to the liver. HPX is formed by two four-bladed beta-propeller domains, resembling two thick disks that lock together at a 90 degrees angle. The heme is bound between the two beta-propeller domains in a pocket formed by the interdomain linker peptide.HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a 'chronosteric' heme-protein.

Product Category

Recombinant Protein

Target

HPX
Synonyms Hemopexin;Hpx;Hpxn

Gene/Protein ID

P02790

Species

Human

Exact Sequence

Thr24-His462
Molecular Weight The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human HPX is greater than 95% as determined by SEC-HPLC.
Human HPX on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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