• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant human Hemopexin binds > 20 uM PPPIX, resulting in a 50% decrease in the fluorescence signal of human Hemopexin.
  • Amino Acid Range: Thr24-His462
Catalog #HPX-HM101
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

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Human HPX Protein (HPX-HM101) Summary

Product Description

 Recombinant Human HPX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr24-His462.

Product Background

 Hemopexin (HPX) serves as scavenger and transporter of toxic plasma heme to the liver. HPX is formed by two four-bladed beta-propeller domains, resembling two thick disks that lock together at a 90 degrees angle. The heme is bound between the two beta-propeller domains in a pocket formed by the interdomain linker peptide.HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a 'chronosteric' heme-protein.

Human HPX Protein (HPX-HM101) Specifications/Details

Protein
 HPX
Synonyms
 Hemopexin;Hpx;Hpxn
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Thr24-His462
Molecular Weight
 The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant human Hemopexin binds > 20 uM PPPIX, resulting in a 50% decrease in the fluorescence signal of human Hemopexin.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human HPX on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HPX on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HPX is greater than 95% as determined by SEC-HPLC. The purity of Human HPX is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...