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Human HRG/HPRG-Protein (HRG-HM101) Summary
Product Description |
Recombinant Human HRG/HPRG-Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val19-Lys525. |
Product Background |
Histidine-rich glycoprotein (HRG) is a 75 kDa glycoprotein synthesized in the liver whose plasma concentration is 100-150 ug/ml. HRG has been shown to modulate sepsis-related biological reactions by binding to several substances and cells, including heparin, factor XII, fibrinogen, thrombospondin, plasminogen, C1q, IgG, heme, LPS, dead cells, bacteria, and fungi. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human HRG/HPRG-Protein (HRG-HM101) Specifications/Details
Protein |
HRG/HPRG |
Synonyms |
HRG; HPRG; THPH11 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val19-Lys525 |
Molecular Weight |
The protein has a predicted MW of 58.75 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human HRG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human HRG is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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