Name: Human HVEM/TNFRSF14 Protein (HVE-HM111)
Brand: KactusBio
SKU: HVE-HM111-100UG
Price: 280.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human HVEM, His Tag at 5ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 84.6 ng/ml determined by ELISA (QC Test).
Amino Acid Range: Leu39-Val202
Other Available Proteins:

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Catalog #HVE-HM111

Size

100UG

500UG

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Human HVEM/TNFRSF14 Protein (HVE-HM111) Summary

Product Description	Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Leu39-Val202.
Product Background	Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human HVEM/TNFRSF14 Protein (HVE-HM111) Specifications/Details

Protein	HVEM/TNFRSF14
Synonyms	CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2
Accession	Q92956-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu39-Val202
Molecular Weight	The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human HVEM, His Tag at 5ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 84.6 ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HVEM, His Tag at 5ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 84.6 ng/ml determined by ELISA (QC Test).
	Immobilized Human HVEM, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA.
	Immobilized Human HVEM, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA.
	Human HVEM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human HVEM, His Tag on Anti-His-Biosensor can bind Human BTLA, hFc (Cat. BTL-HM201) Tag with an affinity constant of 3.67 nM as determined in BLI assay .

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