Product Description |
Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Leu39-Val202. |
Product Background |
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. |
Product Category |
Recombinant Protein |
Target |
HVEM/TNFRSF14 |
Synonyms |
CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu39-Val202 |
Molecular Weight |
The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HVEM, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17 ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE; >95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human HVEM, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17 ug/ml determined by ELISA (QC Test). |
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Immobilized Human HVEM, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA. |
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Immobilized Human HVEM, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. |
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Human HVEM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human HVEM is greater than 95% as determined by SEC-HPLC. |
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