Human HVEM/TNFRSF14 Protein (HVE-HM111)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Leu39-Val202

Catalog: HVE-HM111

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Product Description Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu39-Val202.

Product Background

Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.- Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.

Product Category

Recombinant Protein

Target

HVEM/TNFRSF14
Synonyms CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2

Gene/Protein ID

Q92956-1

Species

Human

Exact Sequence

Leu39-Val202
Molecular Weight The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
The purity of Human HVEM is greater than 95% as determined by SEC-HPLC.
Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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