Human HVEM/TNFRSF14 Protein (HVE-HM111)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.93ug/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Leu39-Val202

Catalog: HVE-HM111

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Product Description	Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu39-Val202.
Product Category	Recombinant Protein

Target	HVEM/TNFRSF14
Synonyms	CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2
Gene/Protein ID	Q92956-1
Species	Human
Exact Sequence	Leu39-Val202
Molecular Weight	The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.93ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.93ug/ml determined by ELISA (QC Test).
	Immobilized Human HVEM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 19.9ng/ml determined by ELISA.
	Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
	The purity of Human HVEM is greater than 95% as determined by SEC-HPLC.
	Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.