Human HVEM/TNFRSF14 Protein (HVE-HM111)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 23.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu39-Val202

Catalog: HVE-HM111

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Product Description Recombinant Human HVEM/TNFRSF14 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Leu39-Val202.
Product Category Recombinant Protein
Target HVEM/TNFRSF14
Synonyms CD270; CD40-like protein;   HVEM; LIGHTR; ATAR; TNFRSF14; TR2
Gene/Protein ID Q92956-1
Species Human
Exact Sequence Leu39-Val202
Molecular Weight The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 23.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 23.1ng/ml determined by ELISA.
Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.44μg/ml determined by ELISA.
Immobilized Human HVEM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
The purity of Human HVEM is greater than 95% as determined by SEC-HPLC.
Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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