Human HVEM/TNFRSF14 Protein (HVE-HM211)
Human HVEM/TNFRSF14 Protein (HVE-HM211)
Human HVEM/TNFRSF14 Protein (HVE-HM211)
Human HVEM/TNFRSF14 Protein (HVE-HM211)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human LIGHT, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3 ng/ml determined by ELISA. The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu39-Val202
Catalog #HVE-HM211
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Product Description
 Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu39-Val202.
Product Background
 Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
Product Category
 Recombinant Protein
Target
 HVEM/TNFRSF14
Synonyms
 CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu39-Val202
Molecular Weight
 The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LIGHT, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3 ng/ml determined by ELISA. The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 uM as determined in SPR assay (Biacore T200).
Immobilized Human LIGHT, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3 ng/ml determined by ELISA.
Immobilized Human BTLA, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5 ng/ml determined by ELISA (QC Test).
Human HVEM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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