Name: Human HVEM/TNFRSF14 Protein (HVE-HM211)
Brand: KactusBio
SKU: HVE-HM211-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: >95% as determined by Bis-Tris PAGE
Activity: Immobilized Human BTLA, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5 ng/ml determined by ELISA (QC Test). The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu39-Val202

Catalog #HVE-HM211

Size

100UG

500UG

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Product Description	Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu39-Val202.
Product Background	Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	HVEM/TNFRSF14
Synonyms	CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2
Accession	Q92956-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu39-Val202
Molecular Weight	The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BTLA, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5 ng/ml determined by ELISA (QC Test). The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 uM as determined in SPR assay (Biacore T200).
	Immobilized Human BTLA, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5 ng/ml determined by ELISA (QC Test).
	Immobilized Human LIGHT, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3 ng/ml determined by ELISA.
	Human HVEM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.