Human HVEM/TNFRSF14 Protein (HVE-HM211)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human LIGHT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA. The affinity constant of 1.2uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu39-Val202

Catalog: HVE-HM211

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Product Description Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu39-Val202.

Product Background

Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.- Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.

Product Category

Recombinant Protein

Target

HVEM/TNFRSF14
Synonyms CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2

Gene/Protein ID

Q92956-1

Species

Human

Exact Sequence

Leu39-Val202
Molecular Weight The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LIGHT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA. The affinity constant of 1.2uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HVEM, hFc Tag captured on Protein A chip, can bind Human CD160, His Tag with an affinity constant of 1.2uM as determined in a SPR assay (Biacore T200).
Immobilized Human LIGHT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA.
Immobilized Human BTLA, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5ng/ml determined by ELISA (QC Test).
Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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