Human Hyper IL-6 Protein (HIL-HM106)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 5.82 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Pro113-Ala323(sIL-6R) & Val30-Met212(IL-6)
Catalog #HIL-HM106
Regular price $450.00
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Size
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Human Hyper IL-6 Protein (HIL-HM106) Summary

Product Description

Recombinant Human Hyper IL-6 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro113-Ala323 (sIL-6R) and Val30-Met212 (IL-6).

Product Background

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Hyper IL-6 Protein (HIL-HM106) Specifications/Details

Protein
IL-6
Synonyms
IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Pro113-Ala323(sIL-6R) & Val30-Met212(IL-6)
Molecular Weight
The protein has a predicted MW of 47.46 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 5.82 nM as determined in SPR assay (Biacore T200).
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Hyper IL-6, His Tag immobilized on CM5 Chip can bind Human gp130, His Tag (Cat. GP1-HM130) with an affinity constant of 5.82 nM as determined in SPR assay (Biacore T200). Human Hyper IL-6, His Tag immobilized on CM5 Chip can bind Human gp130, His Tag (Cat. GP1-HM130) with an affinity constant of 5.82 nM as determined in SPR assay (Biacore T200).
Human Hyper IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Hyper IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Hyper IL-6 is greater than 95% as determined by SEC-HPLC. The purity of Human Hyper IL-6 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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