Name: Human ICAM-1/CD54 Protein (ICM-HM101)
Brand: KactusBio
SKU: ICM-HM101-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ICAM-1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln28-Glu480

Catalog #ICM-HM101

Size

100UG

500UG

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Product Description	Recombinant Human ICAM-1/CD54 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Glu480.
Product Background	Intercellular adhesion molecule-1 (ICAM-1; CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis.
Product Category	Recombinant Protein / Antibody discovery

Protein	ICAM-1/CD54
Synonyms	ICAM-1; BB2; CD54
Accession	AAH15969
Species	Human
Biotinylated	no
Amino Acid Range	Gln28-Glu480
Molecular Weight	The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ICAM-1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human ICAM-1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
	Human ICAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human ICAM-1 is greater than 95% as determined by SEC-HPLC.