Human ICAM-1/CD54 Protein (ICM-HM101)
Catalog: ICM-HM101
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Product Description | Recombinant Human ICAM-1/CD54 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln28-Glu480. |
Product Category | Recombinant Protein |
Target | ICAM-1/CD54 |
Synonyms | ICAM-1; BB2; CD54 |
Gene/Protein ID | AAH15969 |
Species | Human |
Exact Sequence | Gln28-Glu480 |
Molecular Weight | The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human ICAM-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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