Human ICAM-1/CD54 Protein (ICM-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ICAM-1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln28-Glu480

Catalog: ICM-HM101

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Product Description Recombinant Human ICAM-1/CD54 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Glu480.

Product Background

Intercellular adhesion molecule-1 (ICAM-1; CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis.

Product Category

Recombinant Protein

Target

ICAM-1/CD54
Synonyms ICAM-1; BB2; CD54

Gene/Protein ID

AAH15969

Species

Human

Exact Sequence

Gln28-Glu480
Molecular Weight The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human ICAM-1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ICAM-1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
The purity of Human ICAM-1 is greater than 95% as determined by SEC-HPLC.
Human ICAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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