Human ICOS/CD278 (C136S, C137S) Protein (ICO-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ICOS (C136S, C137S), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu21-Phe141(C136S,C137S)
Catalog #ICO-HM102
/
Size

Order now, ships by:

Product Description
 Recombinant Human ICOS/CD278(C136S, C137S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Phe141(C136S,C137S).
Product Background
 Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Product Category
 Recombinant Protein
Target
 ICOS/CD278 (C136S, C137S)
Synonyms
 AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu21-Phe141(C136S,C137S)
Molecular Weight
 The protein has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ICOS (C136S, C137S), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H2, hFc Tag captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
Immobilized Human ICOS (C136S, C137S), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Human ICOS (C136S,C137S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Recently viewed