Human ICOS/CD278 (C136S, C137S) Protein (ICO-HM102)
Catalog: ICO-HM102
Order now get it on
Product Description | Recombinant Human ICOS/CD278(C136S, C137S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Phe141(C136S,C137S). |
Product Background |
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. |
Product Category |
Recombinant Protein |
Target |
ICOS/CD278 (C136S, C137S) |
Synonyms | AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850 |
Gene/Protein ID |
Q9Y6W8-1 |
Species |
Human |
Exact Sequence |
Glu21-Phe141(C136S,C137S) |
Molecular Weight | The protein has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
You may also be interested in
View more associated products