Name: Human ICOS/CD278 (C136S, C137S) Protein (ICO-HM102)
Brand: KactusBio
SKU: ICO-HM102-100UG
Price: 330.0 USD
Availability: InStock

Human ICOS/CD278 (C136S, C137S) Protein (ICO-HM102)

Human B7-H2, hFc Tag captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).

Immobilized Human ICOS (C136S, C137S), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).

Human ICOS (C136S,C137S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ICOS (C136S, C137S), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu21-Phe141(C136S,C137S)

Catalog #ICO-HM102

Size

100UG

500UG

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Product Description	Recombinant Human ICOS/CD278(C136S, C137S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Phe141(C136S,C137S).
Product Background	Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery

Protein	ICOS/CD278 (C136S, C137S)
Synonyms	AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Accession	Q9Y6W8-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu21-Phe141(C136S,C137S)
Molecular Weight	The protein has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ICOS (C136S, C137S), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human B7-H2, hFc Tag captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
	Immobilized Human ICOS (C136S, C137S), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
	Human ICOS (C136S,C137S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.