Human ICOS/CD278 (C136S, C137S) Protein (ICO-HM102)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu21-Phe141(C136S,C137S)

Catalog: ICO-HM102

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Product Description Recombinant Human ICOS/CD278(C136S, C137S) Protein is expressed from Expi293 with His tag at the C-terminal.It contains Glu21-Phe141(C136S,C137S).
Product Category Recombinant Protein
Target ICOS/CD278 (C136S, C137S)
Synonyms AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Gene/Protein ID Q9Y6W8-1
Species Human
Exact Sequence Glu21-Phe141(C136S,C137S)
Molecular Weight The protein has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H2, hFc Tag captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test).
Human ICOS (C136S,C137S) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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