Human ICOS/CD278 (C136S, C137S) Protein (ICO-HM102)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu21-Phe141(C136S,C137S)

Catalog: ICO-HM102

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Product Description Recombinant Human ICOS/CD278(C136S, C137S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Phe141(C136S,C137S).

Product Background

 Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.

Product Category

 Recombinant Protein

Target

 ICOS/CD278 (C136S, C137S)
Synonyms AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850

Gene/Protein ID

 Q9Y6W8-1

Species

 Human

Exact Sequence

 Glu21-Phe141(C136S,C137S)
Molecular Weight The protein has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H2, hFc Tag captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
Immobilized Human ICOS (C136S, C137S) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test).
Human ICOS (C136S,C137S) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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