Name: Human ICOS/CD278 Protein (ICO-HM201)
Brand: KactusBio
SKU: ICO-HM201-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test). The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Glu21-Phe141

Catalog #ICO-HM201

Size

100UG

500UG

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Human ICOS/CD278 Protein (ICO-HM201) Summary

Product Description	Recombinant Human ICOS/CD278 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu21-Phe141.
Product Background	Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human ICOS/CD278 Protein (ICO-HM201) Specifications/Details

Protein	ICOS/CD278
Synonyms	AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Accession	Q9Y6W8-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu21-Phe141
Molecular Weight	The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test). The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
	Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).
	Immobilized Human ICOS, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ICOS Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
	Human ICOS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human ICOS is greater than 90% as determined by SEC-HPLC.

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