Product Description |
Recombinant Human ICOS/CD278 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Phe141. |
Product Background |
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. |
Product Category |
Recombinant Protein |
Target |
ICOS/CD278 |
Synonyms |
AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu21-Phe141 |
Molecular Weight |
The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test). The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test). |
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Human ICOS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human ICOS is greater than 90% as determined by SEC-HPLC. |
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