Human ICOS/CD278 Protein (ICO-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human B7-H2 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu21-Phe141

Catalog: ICO-HM201

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Product Description Recombinant Human ICOS/CD278 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Glu21-Phe141.
Product Category Recombinant Protein
Target ICOS/CD278
Synonyms AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Gene/Protein ID Q9Y6W8-1
Species Human
Exact Sequence Glu21-Phe141
Molecular Weight The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human B7-H2 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human B7-H2 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 21.7ng/ml determined by ELISA.
Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
The purity of Human ICOS is greater than 95% as determined by SEC-HPLC.
Human ICOS on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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