Human ICOS/CD278 Protein (ICO-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human B7-H2, His Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu21-Phe141

Catalog: ICO-HM201

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Product Description Recombinant Human ICOS/CD278 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Glu21-Phe141.
Product Category Recombinant Protein
Target ICOS/CD278
Synonyms AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Gene/Protein ID Q9Y6W8-1
Species Human
Exact Sequence Glu21-Phe141
Molecular Weight The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human B7-H2, His Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-H2, His Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA.
The purity of Human ICOS is greater than 95% as determined by SEC-HPLC.
Human ICOS on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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