Human ICOS/CD278 Protein (ICO-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human B7-H2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA (QC Test). The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu21-Phe141

Catalog: ICO-HM201

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Product Description Recombinant Human ICOS/CD278 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Phe141.

Product Background

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.

Product Category

Recombinant Protein

Target

ICOS/CD278
Synonyms AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850

Gene/Protein ID

Q9Y6W8-1

Species

Human

Exact Sequence

Glu21-Phe141
Molecular Weight The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human B7-H2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA (QC Test). The affinity constant of 5.42 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-H2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA (QC Test).
The purity of Human ICOS is greater than 95% as determined by SEC-HPLC.
Human ICOS on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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