Human ICOS/CD278 (Q50A) Protein (ICO-HM20M)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu21-Phe141

Catalog: ICO-HM20M

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Product Description	Recombinant Human ICOS/CD278 (Q50A) Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Phe141.
Product Background	Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Product Category	Recombinant Protein

Target	ICOS/CD278 (Q50A)
Synonyms	AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Gene/Protein ID	Q9Y6W8-1
Species	Human
Exact Sequence	Glu21-Phe141
Molecular Weight	The protein has a predicted MW of 40.45 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
	The purity of Human ICOS (Q50A) is greater than 95% as determined by SEC-HPLC.
	Human ICOS (Q50A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.