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Human ICOS/CD278 (Q50A) Protein (ICO-HM20M) Summary
Product Description |
Recombinant Human ICOS/CD278 (Q50A) Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu21-Phe141. |
Product Background |
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human ICOS/CD278 (Q50A) Protein (ICO-HM20M) Specifications/Details
Protein |
ICOS/CD278 |
Synonyms |
AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu21-Phe141 |
Molecular Weight |
The protein has a predicted MW of 40.45 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ICOS (Q50A), hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ICOS Antibody, hFc Avi Tag with the EC50 of 91.0ng/ml determined by ELISA. The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human ICOS (Q50A), hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human ICOS (Q50A), hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ICOS Antibody, hFc Avi Tag with the EC50 of 91.0ng/ml determined by ELISA. |
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Human ICOS (Q50A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human ICOS (Q50A), hFc Tag on ProA-Biosensor can bind Human B7-H2, His Tag with an affinity constant of 0.32 µM as determined in BLI assay . |
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The purity of Human ICOS (Q50A) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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