Human ICOS/CD278 (Q50A) Protein (ICO-HM20M)
Human ICOS/CD278 (Q50A) Protein (ICO-HM20M)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu21-Phe141
Catalog #ICO-HM20M
Regular price $346.50
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Human ICOS/CD278 (Q50A) Protein (ICO-HM20M) Summary

Product Description

Recombinant Human ICOS/CD278 (Q50A) Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Phe141.

Product Background

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human ICOS/CD278 (Q50A) Protein (ICO-HM20M) Specifications/Details

Protein
ICOS/CD278 (Q50A)
Synonyms
AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Glu21-Phe141
Molecular Weight
The protein has a predicted MW of 40.45 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
Human ICOS (Q50A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ICOS (Q50A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ICOS (Q50A) is greater than 95% as determined by SEC-HPLC. The purity of Human ICOS (Q50A) is greater than 95% as determined by SEC-HPLC.
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