Name: Human IFN alpha 1 Protein (IFN-HM2A1)
Brand: KactusBio
SKU: IFN-HM2A1-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Cys24-Glu189

Catalog #IFN-HM2A1

Size

100UG

500UG

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Product Description	Recombinant Human IFN alpha 1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys24-Glu189.
Product Background	IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.
Product Category	Recombinant Protein / Antibody discovery

Protein	IFN alpha 1
Synonyms	Interferon alpha-1/13;IFN-alpha-1/13;Interferon alpha-D;LeIF D; IFNalpha 1; IFN alpha1;IFNA1;IFN-alpha-1;Interferon alpha 1
Accession	P01562
Species	Human
Biotinylated	no
Amino Acid Range	Cys24-Glu189
Molecular Weight	The protein has a predicted MW of 46.14 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200) (QC Test).
	Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200) (QC Test).
	Human IFN alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC.