• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Cys24-Glu189
Catalog #IFN-HM2A1
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Human IFN alpha 1 Protein (IFN-HM2A1) Summary

Product Description

 Recombinant Human IFN alpha 1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Cys24-Glu189.

Product Background

 IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IFN alpha 1 Protein (IFN-HM2A1) Specifications/Details

Protein
 IFN alpha 1
Synonyms
 Interferon alpha-1/13;IFN-alpha-1/13;Interferon alpha-D;LeIF D; IFNalpha 1; IFN alpha1;IFNA1;IFN-alpha-1;Interferon alpha 1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Cys24-Glu189
Molecular Weight
 The protein has a predicted MW of 46.14 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200) (QC Test). Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200) (QC Test).
Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200). Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200).
Human IFN alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IFN alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human IFN alpha/beta R2, His Tag on Anti-His-Biosensor, can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.10 nM as determined in BLI assay (Gator). Loaded Human IFN alpha/beta R2, His Tag on Anti-His-Biosensor, can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.10 nM as determined in BLI assay (Gator).
The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC. The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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