Product Description |
Recombinant Human IFN alpha 1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys24-Glu189. |
Product Background |
IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. |
Product Category |
Recombinant Protein |
Target |
IFN alpha 1 |
Synonyms |
Interferon alpha-1/13;IFN-alpha-1/13;Interferon alpha-D;LeIF D; IFNalpha 1; IFN alpha1;IFNA1;IFN-alpha-1;Interferon alpha 1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys24-Glu189 |
Molecular Weight |
The protein has a predicted MW of 46.14 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200) (QC Test). |
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Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200) (QC Test). |
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Human IFN alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC. |
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