Human IFN gamma/IFNG Protein (IFN-HM40G)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IFN gamma, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln24-Gly161

Catalog: IFN-HM40G

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Product Description Recombinant Human IFN gamma / IFNG Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln24-Gly161.
Product Category Recombinant Protein
Target IFN gamma/IFNG
Synonyms Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma
Gene/Protein ID P01579
Species Human
Exact Sequence Gln24-Gly161
Molecular Weight The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IFN gamma, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IFN gamma, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC.
Human IFN gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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