Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G)
Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G)
Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G)
Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G)
Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
  • Amino Acid Range: Gln24-Gly161
Catalog #IFN-HM40G
Regular price $132.00
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Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G) Summary

Product Description

Recombinant Human IFN gamma / IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Gly161.

Product Background

Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G) Specifications/Details

Protein
IFN gamma/IFNG
Synonyms
Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ; IFN γ
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln24-Gly161
Molecular Weight
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Product Tag
C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC. The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC.
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