Product Description |
Recombinant Human IFN gamma / IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Gly161. |
Product Background |
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. |
Product Category |
Recombinant Protein |
Protein |
IFN gamma/IFNG |
Synonyms |
Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ; IFN γ |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln24-Gly161 |
Molecular Weight |
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. |
|
|
Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC. |
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