Human IFN gamma/IFNG Protein (IFN-HM40G)
Human IFN gamma/IFNG Protein (IFN-HM40G)
Human IFN gamma/IFNG Protein (IFN-HM40G)
Human IFN gamma/IFNG Protein (IFN-HM40G)
Human IFN gamma/IFNG Protein (IFN-HM40G)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln24-Gly161
Catalog #IFN-HM40G
Regular price $120.00
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Product Description
Recombinant Human IFN gamma / IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Gly161.
Product Background
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.
Product Category
Recombinant Protein
Target
IFN gamma/IFNG
Synonyms
Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln24-Gly161
Molecular Weight
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IFN gamma is greater than 95% as determined by SEC-HPLC.

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