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Human IFN gamma/IFNG Protein, Ultra Low Endotoxin (IFN-HM00G-UL) Summary
Product Description |
Recombinant Human IFN gamma/IFNG Protein is expressed from E.coli without tag.It contains Gln24-Gln166. |
Product Background |
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human IFN gamma/IFNG Protein, Ultra Low Endotoxin (IFN-HM00G-UL) Specifications/Details
Protein |
IFN gamma/IFNG |
Synonyms |
Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ; IFN γ |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln24-Gln166 |
Molecular Weight |
The protein has a predicted MW of 16.77 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human IFN gamma, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA (QC Test). |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM Tris (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human IFN gamma, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA (QC Test). |
|
|
Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IFN gamma is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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