Human IFN alpha/beta R1 Protein (IFN-HM4R1)
Catalog: IFN-HM4R1
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| Product Description | Recombinant Human IFN alpha/beta R1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Lys28-Lys436. |
| Product Category | Recombinant Protein |
| Target | IFN alpha/beta R1 |
| Synonyms | AVP; IFN-alpha/beta R1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-R-1; CRF2-1; IFRC |
| Gene/Protein ID | P17181-1 |
| Species | Human |
| Exact Sequence | Lys28-Lys436 |
| Molecular Weight | The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 74-95 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IFNAR1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IFNAR1 Antibody, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 0.12 μM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200). |
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Immobilized Human IFNAR1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IFNAR1 Antibody, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. |
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The purity of Human IFNAR1 is greater than 95% as determined by SEC-HPLC. |
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Human IFNAR1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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