Human IFN alpha/beta R1 Protein (IFN-HM4R1)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IFNAR1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IFNAR1 Antibody, hFc Tag with the EC50 of 0.25ug/ml determined by ELISA. The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Lys28-Lys436

Catalog: IFN-HM4R1

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Product Description	Recombinant Human IFN alpha/beta R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys28-Lys436.
Product Category	Recombinant Protein

Target	IFN alpha/beta R1
Synonyms	AVP; IFN-alpha/beta R1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-R-1; CRF2-1; IFRC
Gene/Protein ID	P17181-1
Species	Human
Exact Sequence	Lys28-Lys436
Molecular Weight	The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 74-95 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IFNAR1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IFNAR1 Antibody, hFc Tag with the EC50 of 0.25ug/ml determined by ELISA. The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
	Immobilized Human IFNAR1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IFNAR1 Antibody, hFc Tag with the EC50 of 0.25ug/ml determined by ELISA.
	The purity of-Human IFNAR1 is greater than 95% as determined by SEC-HPLC.
	Human IFNAR1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.