Human IgE Protein (IGE-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human IgE, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Cys209-Lys428

Catalog: IGE-HM401

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Product Description Recombinant Human IgE Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Cys209-Lys428.

Product Background

Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

Product Category

Recombinant Protein

Target

IgE

Gene/Protein ID

P01854-1

Species

Human

Exact Sequence

Cys209-Lys428
Molecular Weight The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IgE, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IgE, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
Human IgE on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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