- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Gly28-Lys291
Product Description |
Recombinant Human IGFBP-3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly28-Lys291. |
Product Background |
Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
IGFBP-3 |
Synonyms |
IBP3; IGFBP3; IGFBP-3; BP-53 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly28-Lys291 |
Molecular Weight |
The protein has a predicted MW of 29.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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