Human IGFBP-3 Protein (IGF-HM103)
Human IGFBP-3 Protein (IGF-HM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly28-Lys291
Catalog #IGF-HM103
Regular price $223.30
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Product Description

Recombinant Human IGFBP-3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly28-Lys291.

Product Background

Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.

Product Category

Recombinant Protein / Antibody discovery

Protein

IGFBP-3

Synonyms

IBP3; IGFBP3; IGFBP-3; BP-53

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly28-Lys291

Molecular Weight

The protein has a predicted MW of 29.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).
Human IGFBP-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IGFBP-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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