Human IGFBP-3 Protein (IGF-HM103)
Catalog: IGF-HM103
Order now get it on
Product Description | Recombinant Human IGFBP-3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly28-Lys291. |
Product Background |
Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer. |
Product Category |
Recombinant Protein |
Target |
IGFBP-3 |
Synonyms | IBP3; IGFBP3; IGFBP-3; BP-53 |
Gene/Protein ID |
AAH18962 |
Species |
Human |
Exact Sequence |
Gly28-Lys291 |
Molecular Weight | The protein has a predicted MW of 29.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
You may also be interested in
View more associated products