Human IGFBP-3 Protein (IGF-HM103)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly28-Lys291

Catalog: IGF-HM103

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human IGFBP-3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly28-Lys291.
Product Background	Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.
Product Category	Recombinant Protein

Target	IGFBP-3
Synonyms	IBP3; IGFBP3; IGFBP-3; BP-53
Gene/Protein ID	AAH18962
Species	Human
Exact Sequence	Gly28-Lys291
Molecular Weight	The protein has a predicted MW of 29.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).
	Human IGFBP-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.