Human IGFBP-6 Protein (IGF-HM106)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IGFBP-6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag (Cat. IGF-HM202) with the EC50 of 60.1ng/ml determined by ELISA.
  • Amino Acid Range: Arg28-Gly240
Catalog #IGF-HM106
Regular price $330.00
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Size
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Human IGFBP-6 Protein (IGF-HM106) Summary

Product Description

Recombinant Human IGFBP-6 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Arg28-Gly240.

Product Background

Insulin-like growth factor binding protein-6 (IGFBP-6) is a growth inhibitory protein that regulates the availability of insulin-like growth factors (IGFs). Expression of IGFBP-6 is associated with non-proliferative states such as cell differentiation and quiescence. Overexpression of IGFBP-6 inhibits tumor growth by inducing apoptosis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IGFBP-6 Protein (IGF-HM106) Specifications/Details

Protein
IGFBP-6
Synonyms
IGF-binding protein 6; IBP-6; IGFBP6; IBP6
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Arg28-Gly240
Molecular Weight
The protein has a predicted MW of 24.22 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IGFBP-6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag (Cat. IGF-HM202) with the EC50 of 60.1ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IGFBP-6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag (Cat. IGF-HM202) with the EC50 of 60.1ng/ml determined by ELISA. Immobilized Human IGFBP-6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag (Cat. IGF-HM202) with the EC50 of 60.1ng/ml determined by ELISA.
Human IGFBP-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IGFBP-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IGFBP-6 is greater than 95% as determined by SEC-HPLC. The purity of Human IGFBP-6 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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