Human IGFBP-7 Protein (IGF-HM207)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD93, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFBP-7, hFc Tag with the EC50 of 0.80ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser27-Leu282

Catalog: IGF-HM207

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Product Description	Recombinant Human IGFBP-7 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Ser27-Leu282.
Product Background	IGFBP-7, also known as Mac25/Angiomodulin (AGM), GFBP-rp1, tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. IGFBP-7 binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
Product Category	Recombinant Protein

Target	Q16270
Accession	IGFBP-7
Species	Human
Amino Acid Range	Ser27-Leu282
Molecular Weight	The protein has a predicted MW of 53.20 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD93, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFBP-7, hFc Tag with the EC50 of 0.80ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD93, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFBP-7, hFc Tag with the EC50 of 0.80ug/ml determined by ELISA.
	The purity of Human IGFBP-7 is greater than 95% as determined by SEC-HPLC.
	Human IGFBP-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.