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Human IGFBP-7 Protein, Ultra Low Endotoxin (IGF-HM2BP-UL) Summary
Product Description |
Recombinant Human IGFBP-7 Protein Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser27-Leu282. |
Product Background |
IGFBP-7, also known as Mac25/Angiomodulin (AGM), GFBP-rp1, tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. IGFBP-7 binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human IGFBP-7 Protein, Ultra Low Endotoxin (IGF-HM2BP-UL) Specifications/Details
Protein |
IGFBP-7 |
Synonyms |
IGFBP-7; IBP7; AGM; FSTL2; IGFBP-7v; IGFBPRP1; MAC25; PSF; RAMSVPS; TAF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser27-Leu282 |
Molecular Weight |
The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IGFBP-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human IGFBP-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA. |
|
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Human IGFBP-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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