• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IGFBP-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA.
  • Amino Acid Range: Ser27-Leu282
Catalog #IGF-HM2BP-UL
Price
Regular price $24563
Regular price Sale price $24563
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Size
Avantor VWR Fisher Scientific

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Human IGFBP-7 Protein, Ultra Low Endotoxin (IGF-HM2BP-UL) Summary

Product Description

 Recombinant Human IGFBP-7 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser27-Leu282.

Product Background

 IGFBP-7, also known as Mac25/Angiomodulin (AGM), GFBP-rp1, tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. IGFBP-7 binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.

Human IGFBP-7 Protein, Ultra Low Endotoxin (IGF-HM2BP-UL) Specifications/Details

Protein
 IGFBP-7
Synonyms
 IGFBP-7; IBP7; AGM; FSTL2; IGFBP-7v; IGFBPRP1; MAC25; PSF; RAMSVPS; TAF;IGFBP7; IGFBP7v
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser27-Leu282
Molecular Weight
 The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IGFBP-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IGFBP-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA. Immobilized Human IGFBP-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA.
Human IGFBP-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IGFBP-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
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