• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IGF1R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 0.83ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly49-Ala118
Catalog #IGF-HM201
Price
Regular price $16500
Regular price Sale price $16500
/
Size
Avantor VWR Fisher Scientific

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Human IGFI Protein (IGF-HM201) Summary

Product Description

 Recombinant Human IGFI Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Gly49-Ala118.

Product Background

 The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes.

Human IGFI Protein (IGF-HM201) Specifications/Details

Protein
 IGFI
Synonyms
 IGF-I; MGF; IBP1; IGF1A;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly49-Ala118
Molecular Weight
 The protein has a predicted MW of 33.18 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IGF1R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 0.83ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
Immobilized Human IGF1R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 0.83ug/ml determined by ELISA (QC Test). Immobilized Human IGF1R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 0.83ug/ml determined by ELISA (QC Test).
Human IGFI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IGFI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IGFI is greater than 95% as determined by SEC-HPLC. The purity of Human IGFI is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...