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Human IGFI Protein, Ultra Low Endotoxin (IGF-HE001-UL) Summary
Product Description |
Recombinant Human IGFI Protein is expressed from E.coli without tag.It contains Gly49-Ala118. |
Product Background |
The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human IGFI Protein, Ultra Low Endotoxin (IGF-HE001-UL) Specifications/Details
Protein |
IGFI |
Synonyms |
IGF-I; MGF; IBP1; IGF1A; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly49-Ala118 |
Molecular Weight |
The protein has a predicted MW of 7.65 kDa. The protein migrates to 8-12 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 8.70 nM as determined in SPR assay (Biacore T200). |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human IGF1R, His Tag captured on CM5 Chip via Protein A can bind Human IGFI, No Tag with an affinity constant of 8.70 nM as determined in SPR assay (Biacore T200). |
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Human IGFI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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