Name: Human IGFII Protein (IGF-HM202)
Brand: KactusBio
SKU: IGF-HM202-100UG
Price: 165.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IGFBP-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 78.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala25-Glu91

Catalog #IGF-HM202

Size

100UG

500UG

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Product Description	Recombinant Human IGFII Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala25-Glu91.
Product Background	The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins. All three ligands are involved in cell proliferation, apoptosis, protein synthesis and metabolism due to their homologous sequences and structural similarities. Insulin-like growth factor 2, a member of the insulin family, plays an important role in embryonic development, metabolic disorders, and tumorigenesis by combining with three receptors with different degrees of affinity.
Product Category	Recombinant Protein / Antibody discovery

Protein	IGFII
Synonyms	IGF-II; Somatomedin-A; Somatomedin A; IGF2; PP1446
Accession	P01344-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala25-Glu91
Molecular Weight	The protein has a predicted MW of 33.00 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IGFBP-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 78.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IGFBP-3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 78.0ng/ml determined by ELISA (QC Test).
	Human IGFII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IGFII is greater than 95% as determined by SEC-HPLC.