Human IGFII Protein (IGF-HM202)

Immobilized Human IGFBP-3, His Tag (Cat. IGF-HM103) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.

Immobilized Human IGFBP-6, His Tag (Cat. IGF-HM106) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag with the EC50 of 60.1ng/ml determined by ELISA.

Human IGFII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IGFII is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IGFBP-3, His Tag (Cat. IGF-HM103) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
Amino Acid Range: Ala25-Glu91

Catalog #IGF-HM202

Price

$181⁵⁰

Size

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Human IGFII Protein (IGF-HM202) Summary

Product Description

Recombinant Human IGFII Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ala25-Glu91.

Product Background

The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins. All three ligands are involved in cell proliferation, apoptosis, protein synthesis and metabolism due to their homologous sequences and structural similarities. Insulin-like growth factor 2, a member of the insulin family, plays an important role in embryonic development, metabolic disorders, and tumorigenesis by combining with three receptors with different degrees of affinity.

Human IGFII Protein (IGF-HM202) Specifications/Details

Protein	IGFII
Synonyms	IGF-II; Somatomedin-A; Somatomedin A; IGF2; PP1446;Insulin-like growth factor-2; Insulin-like growth factor 2
Accession	P01344-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala25-Glu91
Molecular Weight	The protein has a predicted MW of 33.00 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IGFBP-3, His Tag (Cat. IGF-HM103) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
Product Tag	N-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human IGFBP-3, His Tag (Cat. IGF-HM103) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
	Immobilized Human IGFBP-6, His Tag (Cat. IGF-HM106) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGFII, hFc Tag with the EC50 of 60.1ng/ml determined by ELISA.
	Human IGFII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IGFII is greater than 95% as determined by SEC-HPLC.