Human IGFII Protein (IGF-HM202)
Human IGFII Protein (IGF-HM202)
Human IGFII Protein (IGF-HM202)
Human IGFII Protein (IGF-HM202)
Human IGFII Protein (IGF-HM202)
Human IGFII Protein (IGF-HM202)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala25-Glu91
Catalog #IGF-HM202
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Product Description
 Recombinant Human IGFII Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala25-Glu91.
Product Background
 The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins. All three ligands are involved in cell proliferation, apoptosis, protein synthesis and metabolism due to their homologous sequences and structural similarities. Insulin-like growth factor 2, a member of the insulin family, plays an important role in embryonic development, metabolic disorders, and tumorigenesis by combining with three receptors with different degrees of affinity.
Product Category
 Recombinant Protein
Target
 IGFII
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala25-Glu91
Molecular Weight
 The protein has a predicted MW of 33.00 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
Product Tag
 N-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA.
Human IGFII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IGFII is greater than 95% as determined by SEC-HPLC.
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