Human IgG4 Fc Protein (IGG-HM004)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 1.365 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu99-Gly326

Catalog: IGG-HM004

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Product Description	Recombinant Human IgG4 Fc Protein is expressed from HEK293 without tag.It contains Glu99-Gly326.
Product Category	Recombinant Protein

Target	IgG4 Fc
Synonyms	IGHG4
Gene/Protein ID	P01861
Species	Human
Exact Sequence	Glu99-Gly326
Molecular Weight	The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 1.365 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 uM as determined in SPR assay (Biacore T200).
	The purity of Human IgG4 Fc is greater than 95% as determined by SEC-HPLC.
	Human IgG4 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.