Human IgG4 Fc Protein (IGG-HM004) Summary
Product Description |
Recombinant Human IgG4 Fc Protein is expressed from HEK293 without tag.It contains Glu99-Gly326. |
Product Background |
It is known as a IgG4-related disease and its differentiation is based on the analysis of IgG4 levels in the affected tissues. The IgG4-related disease is considered to be a generalized pathological process involving a wide spectrum of various disorders that may affect distant organs. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Human IgG4 Fc Protein (IGG-HM004) Specifications/Details
Protein |
IgG4 Fc |
Synonyms |
IGHG4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu99-Gly326 |
Molecular Weight |
The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.365 uM as determined in SPR assay (Biacore T200). |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 uM as determined in SPR assay (Biacore T200). |
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Human IgG4 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IgG4 Fc is greater than 95% as determined by SEC-HPLC. |
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