Human IgG4 Fc Protein (IGG-HM004)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.365 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu99-Gly326
Catalog #IGG-HM004
Regular price $75.00
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Product Description
Recombinant Human IgG4 Fc Protein is expressed from HEK293 without tag.It contains Glu99-Gly326.
Product Background
It is known as a IgG4-related disease and its differentiation is based on the analysis of IgG4 levels in the affected tissues. The IgG4-related disease is considered to be a generalized pathological process involving a wide spectrum of various disorders that may affect distant organs.
Product Category
Recombinant Protein
Target
IgG4 Fc
Synonyms
IGHG4
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Glu99-Gly326
Molecular Weight
The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 1.365 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
No Tag
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 uM as determined in SPR assay (Biacore T200).
Human IgG4 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IgG4 Fc is greater than 95% as determined by SEC-HPLC.

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