Product Description |
Recombinant Human IgG4 Fc Protein is expressed from HEK293 without tag.It contains Glu99-Gly326. |
Product Background |
It is known as a IgG4-related disease and its differentiation is based on the analysis of IgG4 levels in the affected tissues. The IgG4-related disease is considered to be a generalized pathological process involving a wide spectrum of various disorders that may affect distant organs. |
Product Category |
Recombinant Protein |
Target |
IgG4 Fc |
Synonyms |
IGHG4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu99-Gly326 |
Molecular Weight |
The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.365 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 uM as determined in SPR assay (Biacore T200). |
|
|
Human IgG4 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IgG4 Fc is greater than 95% as determined by SEC-HPLC. |
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