Human IgG4 Fc Protein (IGG-HM004)

  • Express System: Expi293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.365 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu99-Gly326

Catalog: IGG-HM004

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Product Description Recombinant Human IgG4 Fc Protein is expressed from Expi293 without tag.It contains Glu99-Gly326.
Product Category Recombinant Protein
Target IgG4 Fc
Synonyms IGHG4
Gene/Protein ID P01861
Species Human
Exact Sequence Glu99-Gly326
Molecular Weight The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 1.365 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 μM as determined in SPR assay (Biacore T200).
The purity of Human IgG4 Fc is greater than 95% as determined by SEC-HPLC.
Human IgG4 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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