SPR sensorgram showing real-time binding response curves at multiple analyte concentrations for kinetic analysis.
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 14.44 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Leu23-Gly241
Catalog #ISF-HM111
Regular price $297.00
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Human VSIG3/IGSF11 Protein (ISF-HM111) Summary

Product Description

Recombinant Human VSIG3/IGSF11 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly241.

Product Background

Immunoglobulin superfamily member 11 (IgSF11), a homophilic adhesion molecule that preferentially expressed in the brain, is a dual-binding partner of the postsynaptic scaffolding protein PSD-95 and AMPA glutamate receptors (AMPARs).

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human VSIG3/IGSF11 Protein (ISF-HM111) Specifications/Details

Protein
VSIG3/IGSF11
Synonyms
IgSF11; Bt-IGSF; Bt IGSF; BtIGSF; VSIG3; CXADRL1; CT119
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu23-Gly241
Molecular Weight
The protein has a predicted MW of 24.31 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 14.44 uM as determined in SPR assay (Biacore T200).
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human VSIG3, His Tag with an affinity constant of 14.44 uM as determined in SPR assay (Biacore T200). Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human VSIG3, His Tag with an affinity constant of 14.44 uM as determined in SPR assay (Biacore T200).
Human VSIG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VSIG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VSIG3 is greater than 95% as determined by SEC-HPLC. The purity of Human VSIG3 is greater than 95% as determined by SEC-HPLC.
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