Name: Human IL-1 alpha/IL-1A Protein (IL1-HE00A)
Brand: KactusBio
SKU: IL1-HE00A-100UG
Price: 346.5 USD
Availability: InStock

HPLC chromatogram showing a sharp peak at 9.702 minutes, used for product validation of a recombinant protein sample, indicating high purity.

Human IL-1 alpha/IL-1A Protein (IL1-HE00A)

Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).

Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ser113-Ala271

Catalog #IL1-HE00A

Size

100UG

500UG

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Human IL-1 alpha/IL-1A Protein (IL1-HE00A) Summary

Product Description	Recombinant Human IL-1 alpha/IL-1A Protein is expressed from E.coli without tag.It contains Ser113-Ala271.
Product Background	The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IL-1 alpha/IL-1A Protein (IL1-HE00A) Specifications/Details

Protein	IL-1 alpha/IL-1A
Synonyms	Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1
Accession	NP_000566.3
Species	Human
Biotinylated	no
Amino Acid Range	Ser113-Ala271
Molecular Weight	The protein has a predicted MW of 18 kDa same as Bis-Tris PAGE result.
Activity	The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.05 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
	Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.