Human IL-1 alpha/IL-1A Protein (IL1-HM20A)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe113-Thr271

Catalog: IL1-HM20A

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Product Description Recombinant Human IL-1 alpha/IL-1A Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Phe113-Thr271.

Product Background

The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.

Product Category

Recombinant Protein

Target

IL-1 alpha/IL-1A

Gene/Protein ID

P01583

Species

Human

Exact Sequence

Phe113-Thr271
Molecular Weight The protein has a predicted MW of 43.99 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-1R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-1 alpha, hFc Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.
Human IL-1 alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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