Name: Human IL-1 alpha/IL-1A Protein (IL1-HM20A)
Brand: KactusBio
SKU: IL1-HM20A-100UG
Price: 270.0 USD
Availability: InStock

Human IL-1 alpha/IL-1A Protein (IL1-HM20A)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Phe113-Thr271

Catalog #IL1-HM20A

Size

100UG

500UG

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Product Description	Recombinant Human IL-1 alpha/IL-1A Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Phe113-Thr271.
Product Background	The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.
Product Category	Recombinant Protein

Target	IL-1 alpha/IL-1A
Accession	P01583
Species	Human
Biotinylated	no
Amino Acid Range	Phe113-Thr271
Molecular Weight	The protein has a predicted MW of 43.99 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-1R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-1 alpha, hFc Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
	Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.

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