Human IL-1 Rrp2/IL-1 R6 Protein (IL1-HM2L2)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-1 Rrp-2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test). The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asp20-Arg335

Catalog: IL1-HM2L2

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Product Description Recombinant Human IL-1 Rrp2/IL-1 R6 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp20-Arg335.

Product Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9). IL1RL2 is a receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways.

Product Category

Recombinant Protein

Target

IL-1 Rrp2/IL-1 R6
Synonyms IL-1 R6; IL-1 Rrp2; IL1RL2; IL1Rrp2; IL1RRP2; IL-36R

Gene/Protein ID

Q9HB29-1

Species

Human

Exact Sequence

Asp20-Arg335
Molecular Weight The protein has a predicted MW of 62.6 kDa. Due to glycosylation, the protein migrates to 75-110 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-1 Rrp-2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test). The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
Immobilized Human IL-1 Rrp-2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test).
The purity of Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC.
Human IL-1 Rrp2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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