• Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ser19-Asn178
Catalog #IL1-HM010
Price
Regular price $412.50
Regular price Sale price $412.50
/
Size
Avantor VWR Fisher Scientific

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Human IL-10 Protein (IL1-HM010) Summary

Product Description

 Recombinant Human IL-10 Protein is expressed from HEK293 without tag.It contains Ser19-Asn178.

Product Background

 Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IL-10 Protein (IL1-HM010) Specifications/Details

Protein
 IL-10
Synonyms
 Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser19-Asn178
Molecular Weight
 The protein has a predicted MW of 18.8 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).
Product Tag
 No Tag

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200). Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test). Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test).
Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL (QC Test). Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL (QC Test).
The purity of Human IL-10 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-10 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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