Product Description |
Recombinant Human IL-10 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ser19-Asn178. |
Product Background |
Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. |
Product Category |
Recombinant Protein |
Target |
IL-10 |
Synonyms |
Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser19-Asn178 |
Molecular Weight |
The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-10, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-10, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA (QC Test). |
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Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-10 is greater than 95% as determined by SEC-HPLC. |
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