Human IL-10 Protein (IL1-HM410)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-10, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser19-Asn178

Catalog: IL1-HM410

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Product Description	Recombinant Human IL-10 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ser19-Asn178.
Product Category	Recombinant Protein

Target	IL-10
Synonyms	Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451
Gene/Protein ID	P22301
Species	Human
Exact Sequence	Ser19-Asn178
Molecular Weight	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-10, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-10, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA.
	The purity of Human IL-10 is greater than 95% as determined by SEC-HPLC.
	Human IL-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.