Human IL-10 Protein (IL1-HM410)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser19-Asn178

Catalog: IL1-HM410

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Product Description Recombinant Human IL-10 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Ser19-Asn178.
Product Category Recombinant Protein
Target IL-10
Synonyms Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451
Gene/Protein ID P22301
Species Human
Exact Sequence Ser19-Asn178
Molecular Weight The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
The purity of Human IL-10 Protein is greater than 95% as determined by SEC-HPLC.
Human IL-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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