Human IL-11 Protein (IL1-HM111)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Activity: Immobilized Human IL-11, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Pro22-Leu199

Catalog: IL1-HM111

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Product Description	Recombinant Human IL-11 Protein is expressed from HEK293 with His tag at the N-Terminus; It contains Pro22-Leu199.
Product Background	Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers, human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.
Product Category	Recombinant Protein

Target	IL-11
Synonyms	Interleukin-11;IL11;Adipogenesis inhibitory factor;AGIF;INN: Oprelvekin;Oprelvekin;IL-11Oprelvekin;interleukin 11;interleukin-11
Accession	P20809-1
Species	Human
Amino Acid Range	Pro22-Leu199
Molecular Weight	The protein has a predicted MW of 20.24 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-11, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind Human IL-11, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-11, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA (QC Test).
	The purity of Human IL-11 is greater than 95% as determined by SEC-HPLC.
	Human IL-11 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.