• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-11, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Pro22-Leu199
Catalog #IL1-HM111
Price
Regular price $198.00
Regular price Sale price $198.00
/
Size
Avantor VWR Fisher Scientific

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Human IL-11 Protein (IL1-HM111) Summary

Product Description

 Recombinant Human IL-11 Protein is expressed from HEK293 with His tag at the N-Terminus; It contains Pro22-Leu199.

Product Background

 Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers, human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IL-11 Protein (IL1-HM111) Specifications/Details

Protein
 IL-11
Synonyms
 Interleukin-11;IL11;Adipogenesis inhibitory factor;AGIF;INN: Oprelvekin;Oprelvekin;IL-11Oprelvekin;interleukin 11;interleukin-11
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro22-Leu199
Molecular Weight
 The protein has a predicted MW of 20.24 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-11, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM Citric acid (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 20mM Citric acid (pH 3.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind Human IL-11, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind Human IL-11, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-11, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA (QC Test). Immobilized Human IL-11, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA (QC Test).
Human IL-11 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-11 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF-1 cells. The ED50-for this effect is typically 1-8 ng/mL. Measured in a cell proliferation assay using TF-1 cells. The ED50-for this effect is typically 1-8 ng/mL.
Other Available Proteins:
Finding available proteins...
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