Human IL-11 Protein (IL1-HM111)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-11, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 26.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Pro22-Leu199

Catalog: IL1-HM111

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Product Description Recombinant Human IL-11 Protein is expressed from Expi293 with His tag at the N-terminal.It contains Pro22-Leu199.
Product Category Recombinant Protein
Target IL-11
Synonyms Interleukin-11;IL11;Adipogenesis inhibitory factor;AGIF;INN: Oprelvekin;Oprelvekin;IL-11Oprelvekin;interleukin 11;interleukin-11
Gene/Protein ID P20809-1
Species Human
Exact Sequence Pro22-Leu199
Molecular Weight The protein has a predicted MW of 20.24 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-11, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 26.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind Human IL-11, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-11, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 26.2ng/ml determined by ELISA (QC Test).
The purity of Human IL-11 is greater than 95% as determined by SEC-HPLC.
Human IL-11 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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