Human IL-11R alpha Protein (ILR-HM111) Summary
Product Description |
Recombinant Human IL-11R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser24-Ala370. |
Product Background |
Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers,human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human IL-11R alpha Protein (ILR-HM111) Specifications/Details
Protein |
IL-11R alpha |
Synonyms |
IL-11R subunit alpha; IL-11R-alpha; IL-11RA; IL11RA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser24-Ala370 |
Molecular Weight |
The protein has a predicted MW of 38.8 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind Human IL-11, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). |
|
|
Human IL-11R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IL-11R alpha is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
