Human IL-12 Protein (IL1-HM412)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
  • Activity: Immobilized Human IL-12 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile23-Ser328 (IL-12B)/Arg23-Ser219 (IL-12A)

Catalog: IL1-HM412

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Product Description Recombinant Human IL-12 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Ile23-Ser328 (IL-12B)/Arg23-Ser219 (IL-12A).
Product Category Recombinant Protein
Target IL-12
Synonyms CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12
Gene/Protein ID P29460 (IL-12B)&P29459 (IL-12A)
Species Human
Exact Sequence Ile23-Ser328 (IL-12B)/Arg23-Ser219 (IL-12A)
Molecular Weight The protein has a predicted MW of 34.7 (IL-12B)/25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-12 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-12 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA.
The purity of Human IL-12 is greater than 95% as determined by SEC-HPLC.
Human IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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