Human IL-12 Protein (IL1-HM412)
Human IL-12 Protein (IL1-HM412)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
  • Activity: Immobilized Human IL-12, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
Catalog #IL1-HM412
Regular price $525.00
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Product Description
Recombinant Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328 (IL-12B) & Arg23-Ser219 (IL-12A).
Product Background
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Product Category
Recombinant Protein
Target
IL-12
Synonyms
CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
Molecular Weight
The protein has a predicted MW of 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-12, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-12, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).
Human IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 0.1 - 1 ng/mL.
The purity of Human IL-12 is greater than 95% as determined by SEC-HPLC.

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