Human IL-12 R beta 1/CD212 Protein (ILR-HM212)
Human IL-12 R beta 1/CD212 Protein (ILR-HM212)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Cys24-Glu540
Catalog #ILR-HM212
Regular price $247.50
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Product Description

Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Cys24-Glu540.

Product Background

Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

IL-12 R beta 1/CD212

Synonyms

IL-12R-beta-1; IL-12RB1; IL12R; IL12RB; CD212; IL-12R subunit beta-1; IL-12R-BETA1; MGC34454;IL-12R-β-1; IL-12R subunit β-1; IL-12R-β1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Cys24-Glu540

Molecular Weight

The protein has a predicted MW of 83.85 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA.
Human IL-12 R beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-12 R beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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