Product Description |
Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Cys24-Glu540. |
Product Background |
Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function). |
Product Category |
Recombinant Protein |
Target |
IL-12 R beta 1/CD212 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys24-Glu540 |
Molecular Weight |
The protein has a predicted MW of 83.85 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. |
|
|
Human IL-12 R beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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