Human IL-12B/p40/NKSF2 Protein, Ultra Low Endotoxin (P40-HM112-UL)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). The affinity constant of 98.1nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ile23-Ser328

Catalog #P40-HM112-UL

Price

$327.00

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Human IL-12B/p40/NKSF2 Protein, Ultra Low Endotoxin (P40-HM112-UL) Summary

Product Description	Recombinant Human IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Ser328.
Product Background	Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-12B/p40/NKSF2
Synonyms	IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS
Accession	P29460
Species	Human
Biotinylated	no
Amino Acid Range	Ile23-Ser328
Molecular Weight	The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). The affinity constant of 98.1nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
	Immobilized Human IL-12B, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test).
	Immobilized Human IL-12B, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA.
	Human IL-12B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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