Human IL-13 Protein (IL1-HM213)
Catalog: IL1-HM213
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Product Description | Recombinant Human IL-13 Protein is expressed from Expi293 with hFc tag at the N-terminal.It contains Gly21-Asn132. |
Product Category | Recombinant Protein |
Target | IL-13 |
Synonyms | IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789 |
Gene/Protein ID | AAK53823 |
Species | Human |
Exact Sequence | Gly21-Asn132 |
Molecular Weight | The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human IL-13, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra1, His Tag with the EC50 of 0.83μg/ml determined by ELISA (QC Test). The affinity constant of 0.399 μM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | N-hFc |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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