Human IL-13 Protein (IL1-HM213)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly21-Asn132

Catalog: IL1-HM213

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Product Description	Recombinant Human IL-13 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gly21-Asn132.
Product Background	Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Product Category	Recombinant Protein

Target	IL-13
Synonyms	IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Gene/Protein ID	AAK53823
Species	Human
Exact Sequence	Gly21-Asn132
Molecular Weight	The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test).
	The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC.
	Human IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.