Human IL-13 Protein (IL1-HM213)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly21-Asn132

Catalog: IL1-HM213

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Product Description Recombinant Human IL-13 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gly21-Asn132.

Product Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Product Category

Recombinant Protein

Target

IL-13
Synonyms IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Accession

Species

Human

Amino Acid Range

Gly21-Asn132
Molecular Weight The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA (QC Test).
Immobilized Human IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 7.1ng/ml determined by ELISA.
The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC.
Human IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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