Human IL-13 Protein (IL1-HM213)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly21-Asn132
Catalog #IL1-HM213
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Product Description
 Recombinant Human IL-13 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gly21-Asn132.
Product Background
 Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Product Category
 Recombinant Protein
Target
 IL-13
Synonyms
 IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly21-Asn132
Molecular Weight
 The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-hFc
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test).
Immobilized Human IL-13, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 7.1 ng/ml determined by ELISA.
Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC.

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