Human IL-13Ra1 Protein (ILR-HM1R1)
Human IL-13Ra1 Protein (ILR-HM1R1)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.55 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly22-Thr343
Catalog #ILR-HM1R1
Regular price $297.00
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Human IL-13Ra1 Protein (ILR-HM1R1) Summary

Product Description

Recombinant Human IL-13Ra1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly22-Thr343.

Product Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Human IL-13Ra1 Protein (ILR-HM1R1) Specifications/Details

Protein
IL-13Ra1
Synonyms
IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1; AI882074
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly22-Thr343
Molecular Weight
The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.55 uM as determined in SPR assay (Biacore T200).
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-13, No Tag (Cat. IL1-HM013) immobilized on CM5 Chip can bind Human IL-13Ra1, His Tag with an affinity constant of 0.55 uM as determined in SPR assay (Biacore T200). Human IL-13, No Tag (Cat. IL1-HM013) immobilized on CM5 Chip can bind Human IL-13Ra1, His Tag with an affinity constant of 0.55 uM as determined in SPR assay (Biacore T200).
Human IL-13Ra1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-13Ra1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra1, His Tag with an affinity constant of 1.15 µM as determined in BLI assay. Loaded Human IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra1, His Tag with an affinity constant of 1.15 µM as determined in BLI assay.
The purity of Human IL-13Ra1 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-13Ra1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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